Amphiphysin GTPase stimulation

Fig. 1. Dynamin GTPase assay was performed in the presence of truncated amphiphysin. The truncated amphiphysins were designed as shown in (A). Effect of these amphiphysins on dynamin GTPase activity was assayed (B). The data was normalized by the GTPase activity of dynamin. Deletion of the middle domain of amphiphysin (Amph A248-315 and Amph A248-601) resulted in strong stimulation of the dynamin GTPase activity. Binding sites for AP-2 and clathrin are depicted in (A). (Reproduced with permission from Y. Yoshida et al. [2004]. EMBO J. 23, 3483-3491.)...

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