Ammonia from Cisplatin

Release of ammonia from cisplatin inside red cells in vitro on reaction with intracellular glutathione (GSH, 2 mM) can be detected by NMR studies on intact cells (28) initially, displacement of Cl occurs, giving cis-[Pt(SG)(Cl)(NH3)2], and finally a 1 2 Pt GSH polymer. The trans isomer reacts more rapidly, but forms the stable product trans-[Pt(SG)2(NH3)2l. 

Thus, the possible number of aquated forms derived from cA-DDP is great and the proportion dependent on such variations as time, pH, temperature, and concentration of associated reactants (chloride ion and ammonia). Figure 1 contains structures of some of the aquated forms of cisplatin. 

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