Amino-Terminal Residue and Sequence

The use of the method of dinitrophenylation (DNP) (Sanger, 1945) enabled Porter and Sanger (1948) to confirm the presence of proline at the N-terminus of salmine. Since then, many workers have studied N-terminal residues and sequences 

Before these results were obtained, a good many data were accumulated by various classical methods on the presence or absence of N-terminal amino or imino groups in clupeine and salmine (Kossel and Cameron, 1912 Kossel and Gawrilow, 1912 Felix and Dirr, 1929 Felix and Mager, 1937 Felix et al., 1950 Waldschmidt-Leitz et aL 1931 Rasmussen and Linderstrom-Lang, 1934 Linderstrom-Lang, 1935 Tristram, 1949). 

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