Amino sugars analysis, structural

The purification of a pneumococcal polysaccharide may be difficult, and the polysaccharide material is sometimes contaminated by a cell-wall component known as the C-substance. The methods for structural analysis of polysaccharides (which, like several of the pneumococcal polysaccharides, contain amino sugar and uronic acid... 

The application of chromatographic techniques to the analysis of mucopolysaccharides has provided a new impetus in the structural study of these substances and has opened up new fields of research. The behavior of glucosamine on ion-exchange resins was early examined by Freudenberg, Walch and Molter71 and the amino sugar has been separated... 

Full structural analysis of a real cell membrane reveals a chemically diverse thin sheet composed of phospholipid bilayers penetrated by glycoproteins containing the amino sugars we discussed earlier. The amount of each component varies but there is usually about 50 50 phospholipid protein, with the protein containing about 10% sugar residues. The phospholipids main role is as a barrier while the glycoproteins have the roles of recognition and transport. 

Reviews have appeared on the use of high resolution n.m.r. spectroscopy in the structural determination of complex carbohydrates and of some 3-0-, 4-0-, and 3,4-di-O-glycopyranosyl-substituted methyl D-gtycopyranosides. The latter publication includes complete assignments of and C-n.m.r. resonances as well as conformational studies using HSEA effect theory. The application of various njnj. techniques (multi-relayed correlation, H- H-correlated, triple quantum field H- H-correlated and H- H homonuclear Hartmann Hahn spectroscopy) to carbohydrate analysis has been reviewed in Japanese, and in a further Japanese review the interactions of metal ions with uronic acids and amino sugars as observed by the lanthanide-probe n.m.r. method are discussed/... 

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