Amino acid separation by ion exchange

J. B. Kafil and C. O. Huber, Flow Injection Sample Processing with Nickel Oxide Electrode Amperometric Detection of Amino Acids Separated by Ion-Exchange Chromatography. Anal. Chim. Acta, 139 (1982) 347. 

In the 1950s, W. H. Stein and S. Moore conducted pioneering work at Rockefeller Institute to understand the structure of the enzyme ribonuclease, for which they were awarded the Nobel Prize in 1972. Continual need to measure amino acids led them to explore amino acid separations by ion exchange. By 1958, their design led to the first commercial automated amino acid analyzer— a revolutionary advance for biochemistry. 

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