Alumina plasma synthesis

The synthesis of DPE was described by Nohta et al. [411]. After the usual clean-up by alumina adsorption, cation exchange column chromatography or other suitable procedure [412], 150 fil of the sample solution (adjusted to pH 6.0-6.5) are mixed with 10 fil of a 20 mM solution of potassium ferricyanide and 150 1 of acetonitrile. Subsequently, 50 fA of the DPE solution (21.2 mg ml in 0.1 M HCl) are added and the reaction mixture is allowed to stand at 37 °C for 40 min. After cooling to room temperature, 100 /A aliquots are injected onto the column. Fluorescence intensity is recorded at 485 nm after excitation at 345 nm. Procedures for the determination of norepinephrine, epinephrine and dopamine in plasma [412] and urine [413] were reported. 

Fig. 19.2.1. shows the procedure for the fabrication of nano-porous diamond films (diamond nanohoneycomb) by template synthesis with porous alumina membranes. Ordered thorough-hole anodic porous alumina membranes were laid on the top of the synthetic diamond films, and then deep holes were etched into the film by use of an oxygen plasma treatment. 

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