Alkaline phosphatase purification procedures

Porous zirconia particles were also treated with polyphosphates of chain lengths between 3 and 100 for affinity chromatography of nucleic acids and proteins. The affinity matrix was stable in the pH range of 3 to 11 during a 120 hr incubation period. It is demonstrated that polyphosphate-modified zirconia not only is a usefiil support for the separation of cationic proteins as is phosphate-modified zirconia, but also can be used for the binding of neutral proteins (e.g., DNase I) and anionic proteins (e.g., alkaline phosphatase). Applying both HPLC and batch procedures can perform protein separation on this packing. The polyphosphate-modified zirconia can also be used in the effective purification of nucleic acids. It was found that 1) double-stranded DNA does not bind to the zirconia... 

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