Algorithms peak width estimation

In both of the peak width algorithms, it is necessary to estimate the height of the peak at a position which, more likely than not, is between two channels. A full Gaussian fitting over the peak region would provide this information but a simpler, and sufficiently accurate, approach can be used. The channel contents in a Gaussian peak should be related by the equation ... 

Fig. 8.8. By looking at the peak at the 6C and 8C positions (aggregates of three and four cells), software algorithms use this information to estimate the contribution of clumps of two cells to the peak at the G2/M (4C) position. The graph at the right indicates the software estimation of these aggregated cells. The graph at the left indicates where these cells fall on a plot of signal area versus signal width.

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