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Aldehydes or Ketones with AMBH

Dissolve an aldehyde-containing macromolecule to be modified (i.e., a periodate-oxidized glycoprotein) in 0.01M sodium phosphate, 0.15M NaCl, pH 7.4, containing ImM EDTA. A suitable concentration range for a protein is l-10mg/ml. [Pg.84]

Add a 10-fold molar excess of AMBH (pre-dissolved in ethanol) (Molecular Probes) over the expected amounts of aldehydes to be modified. [Pg.84]

See other pages where Aldehydes or Ketones with AMBH is mentioned: [Pg.83] [Pg.91] [Pg.71] [Pg.83] [Pg.91] [Pg.71] [Pg.372] [Pg.83] [Pg.91] [Pg.71]

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Aldehydes or ketones

Aldehydes with ketones

Or ketones