Serum albumin, bovine multilayer protein

Jiang and Zhu (2000) and Qiu and Zhu (2001) have reported the fabrication of multilayered devices composed of stacks of compression-molded disks of alternating compositions. One type of disk is either P(SA-EG) or P[SA-co-TMAgly)-Z>-EG] and the other is a pH-sensitive, protein-loaded blend of, for example, poly(methacrylic acid) and polyethoxazoline. The release of model proteins, myoglobin, bovine serum albumin, and FITC-dextran, and compounds such as brilliant blue have been studied and pulsatile release profiles have been demonstrated (Jiang and Zhu, 2000 Qiu and Zhu, 2001). 

Kent and Meyer (1984) made broad-band dielectric measurements in the frequency range 0.3-16 GHz on various hydrated protein powders, including hemoglobin and bovine serum albumin. Comparison of relaxation spectra measured at 20-80°C suggested that at high hydration there is more than one state of multilayer water. 

In another study, QCM-D provides insight into how a protein interacts with a hydrogel film, e.g., monolayer or multilayer adsorption onto the surface versus penetration into the material. Alf et al. (2011a) show how bovine serum albumin penetrates an expanded thermo-responsive copolymer but forms an adsorbed monolayer when the material is collapsed above the phase transition temperature (Figure 6.11). 

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