Agitation from TCAs

Excessive central stimulation, usually exhibited as tremors, insomnia and hyperhidrosis, can occur following therapeutic doses of the MAOIs, as can agitation and hypomanic episodes. Peripheral neuropathy, which is largely restricted to the hydrazine type of MAOI, is rare and has been attributed to a drug-induced p)n idoxine deficiency. Such side effects as dizziness and vertigo (presumably associated with hypotension), headache, inhibition of ejaculation (which is often also a problem with the TCAs), fatigue, dry mouth and constipation have also been reported. These side effects appear to be more frequently associated with phenelzine use. They are not associated with any antimuscarinic properties of the drug but presumably arise from the enhanced peripheral sympathetic activity which the MAOIs... 

Extraction. Swollen pronuclei are separated from unbound MVs by centrifugation through 1 M sucrose as described in Section V, washed in 0.5 ml TN, and spun at 1500 g for 10 min. Pelleted nuclei are resuspended in 1 ml of 1% TX-100 in TN, and lamins extracted for 1 hr at 4°C with agitation. The chromatin is spun down at 3000 g, proteins from the supernatant precipitated with ice-cold 10% trichloroacetic acid (TCA), the pellet washed twice in cold acetone, and protein solubilized in lx SDS sample buffer. 

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