Affinity nature

Zhang, R.-G., et al. The crystal structure of trp aporepressor at 1.8 A shows how binding tryptophan enhances DNA affinity. Nature 327 591-S97, 1987. 

NC Greenham, SC Moratti, DDC Bradley, RH Friend, and AB Holmes, Efficient light-emitting diodes based on polymers with high electron affinities, Nature, 365 628-630, 1993. 

However, it is possible to add the non-observed component (usually the ligand) in excess (solubility permitting) in order to cope with weak (i.e., pM-mM) binding affinities. Naturally, this requires an even higher performance of the isotope filtering technique used to suppress the (excess) component. 

Gartenberg, M. R. Crothers, D. M. (1988). DNA sequence determinants of CAP-induced bending and protein binding affinity. Nature 333, 824-829. 

M27. Morimoto, H., Lehmann, H., and Perutz, M. F., Molecular pathology of human haemoglobin stereochemical interpretation of abnormal oxygen affinities. Nature (London) 232, 408-413 (1971). 

Veillard [19] covers a similar range of molecules but from the Hartree-Fock and post-HF view. The discussion is organised more in terms of molecular properties. Thus, he deals with metal carbonyls, carbides, cyanides, C02 complexes, alkyls, carbenes, carbynes, alkenes, alkynes and metallocenes under the headings of electronic states, electronic spectra, optimised geometries, binding energies, Ionisation Potentials and Electron Affinities, nature of M-L bonding and other properties (e.g. vibrational spectra, dipole moments and electron distributions). 

N. C. Greenham, S. C. Moratti, D. D. C. Bradley, R. H. Friend, A. B. Holmes, Efficient Light-Emitting Diodes Based on Polymers with High Electron Affinities. Nature 1993, 365, 628-630. 

The affinity chromatography of a-D-acetamidodeoxygalactosidase and j3-d-acetamidodeoxyglucosidase on an agarose derivative that had been allowed to react with diazophenyl oxamate has been investigated. The binding of the enzymes to the matrix, which hitherto has been considered to be specific for neuraminidase, is now thought to be non-specific, and it is doubtful whether the association is of a true affinity nature. 

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