Affinity chromatography risks

New affinity purification techniques involving water-soluble biospecific polymers have been reported during the past 15 years, with the ambition of providing an alternative to affinity chromatography. This approach was prompted owing to certain limitations among which the difficulty to properly control the synthesis of the affinity matrix and the risk of non-specific binding of contaminants are directly related to the use of insoluble supports. 

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