Affinity chromatography inert matrix

Affinity chromatography exploits the specific, high affinity, noncovalent binding of a protein to another molecule, the ligand. First, the ligand is covalently attached to an inert and porous matrix (such as Sepharose). The protein mixture is then... 

Affinity chromatography relies on the ability of enzymes to bind to ligands that are bound to an inert support matrix packed in the column. Thus, only enzymes that selectively bind to ligands will be retained in the column. Unbound enzymes will be flushed out by column washing using an appropriate buffer solution that contains free ligands. 

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