Affinity chromatography elution possibilities

Fig. 3. Pattern of elution of tetramer from 10 ml of the same AU-heterozygous serum as was used in the experiment of Fig. 2, with a longer affinity chromatography column (1 x 30 cm). Assignment of the tetrameric composition of each fraction was based upon the dibu-caine number and total activity, and the calculated dibucaine numbers for each possible tetramer. The homologous tetramers at the beginning and end, A4 and U4, had dibucaine numbers of 15 and 78, respectively. (After La Du and Choi, L2.)...

$Fig. 3. Pattern of elution of tetramer from 10 ml of the same AU-heterozygous serum as was used in the experiment of Fig. 2, with a longer affinity chromatography column (1 x 30 cm). Assignment of the tetrameric composition of each fraction was based upon the dibu-caine number and total activity, and the calculated dibucaine numbers for each possible tetramer. The homologous tetramers at the beginning and end, A4 and U4, had dibucaine numbers of 15 and 78, respectively. (After La Du and Choi, L2.)...$

Fig. 1. The steps of affinity chromatography (a) A bioligand is immobilized (b) A crude extract is prepared and freed from endogenous substrate (c) The substrate-free extract is applied to the chromatographic packing of immobilized bioligand from step (a) (d) Unwanted protein is removed by washing and (e) The desired protein is eluted, possibly with...

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...