Affinity chromatography definition

Absorption, 6, 8, 40 Accuracy, definition of, 99 Activity coefficient, 109-110 Adjusted retention volume, 10 Adsorption, 6, 8, 40 Affinity chromatography, 224-225 Area, measurement of, 102 Area normalization, 104 Asymmetry of peaks, 14, 43... 

Since 1961, when the affinity labeling was introduced into re rch practice, a lot of different affinity labels have been applied The phenomenon of mutual affinity of certain proteins to the other definite compounds has been successfully applied in affinity cytochemistry affinity chromatography and affinity therapy... 

Summarizing the above description, the surface of polyamine graft copolymers with a definite amount of polyamine branches showed an extremely small quantity of non-specifically adsorbed lymphocytes. This advantageous characteristic of polyamine graft copolymers led us to utilize them as solid-phase matrices for cell affinity chromatography. 

The possible formation of a dipole is a feature of covalent bonding but it is obvious that an ionic bond results in a definite unequal distribution of electrons within a molecule and such molecules (or ions) are extremely polar. However, the fact that they carry a definite charge enables additional separation techniques to be applied. The rate of migration in an electric field (electrophoresis) and the affinity for ions of opposite charge (ion-exchange chromatography) are extremely valuable techniques in the separation of ionic species. 

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