Adsorption isotherms Scatchard plot

Fig. 5.7. (A) Scatchard plot of the adsorption isotherm in CHClj of 9EA to a MIP imprinted with 9EA (P9EA) and (B) Scatchard plot of the differential binding to PEA after subtraction of the binding to a MIP imprinted with BA. From Shea et al. [24].

Fig. 8.1. Scatchard (A), Langmuir (B and C) and Sips (D) forms of the Law of Mass Action for antibody-antigen interactions. The abbreviations and the derivation of the equations are given in Section 8.3. Polyclonal sera yield curved lines in A, B and C, whereas the interrupted lines represent homogeneous interaction. In the Scatchard (1949) plot, three of the different populations of antibodies are indicated by dotted lines low afTinity (K is small) at r = 2, intermediate ( average ) affinity, Aoat r = 1 and high affinity at small r. The Langmuir (1918) adsorption isotherm can be graphically presented if the total amount of antibody is known (B) or not (C). In the latter case b represents bound antibody (Abt d) and n/r in eq. 9 is substituted by Abio,ai/Abbo d to obtain ...

Figure 10 Binding isotherm and (inset) Scatchard plot for cholesterol adsorption by the cholesterol-imprinted polymer (10 mg mL ), prepared by the sacrificial spacer method. Reprinted with permission from Journal of the American Chemical Society. Copyright 1995 American Chemical Society (Ref. 10).

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