AdS EVLS of Hairpins

Escherichia coli heat-shock gene, and in rRNA genes. Except spectral and thermodynamic analysis (CD, NMR, and calorimetry), this heptamer was studied electrochemically (CV, LSV, EVLS) [46]. On mercury electrodes the hairpin d(GCGAAGC) provides voltammetric reduction signals of A and C, and oxidation signals of G. Both signals have been studied in dependence on pH, accumulation time, scan rate, and loop sequences. The AdS EVLS was employed for the determination of the detection limit (2 nM), which was verified by multidimensional voltammetric analysis using Fourier transform in combination with the confidence ellipse statistic method. Our results showed the difference in electrochemical behavior of DNA and RNA heptamers (Fig. 11.5). 

While RNA hairpin (Fig. 11.5b) provides one anodic G peak, DNA hairpin gives two G peaks (Fig. 11.5a) whose heights depend on pH. This phenomenon is very interesting because guanine-containing compounds on mercury electrodes provide a single anodic peak G, which corresponds to the oxidation of reduction product generated 

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