Acyl carrier protein fusion proteins

Guerra. 1988. Expression of an active spinach acyl carrier protein-I/protein-A fusion. Plant Molec. Biol, (submitted). 

The protein A acyl carrier protein-I gene was partially sequenced and found to be completely homologous to our desired construction (7). The purified protein from E. coli N4830 cells was approximately 90% pure as judged from SDS PAGE using both Coomassie staining and Western immunoblot detection (data not shown). This purified preparation was stable at -20° for several months and was used as source of fusion AGP for in vitro enzymatic characterizations. 

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