Specific activity, radiolabeled assessment

Most assessments of proteins and/or enzymic activities associated with the cestode surface have not attempted to establish whether the proteins under study originate from the host or parasite. One possible approach for identifying proteins synthesised specifically by parasites is outlined in Fig. 6.5. It involves radiolabelling surface components (494) followed by their fractionation by immunoprecipitation, SDS/PAGE and autoradiography. An alternative approach is that of Atkinson Podesta (30), who radiolabelled adults of H. diminuta with [14C]leucine in vitro. Subsequent one- and two-dimensional gel electrophoretic separation and staining of the... 

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