Activity determination of horseradish peroxidase

The concentration of H2O2 in a 30% solution is not very stable, even when stored at 4°C real concentration can be measured at 240 nm where its molar extinction coefficient is 43.6 M cm (Hilde- 

Total volume is 3.11 ml . 4436 is measured extinction coefficient of guaiacol (tetra-guaiacol is formed) is 25.6/4 cm //imole. 

The volume activity (Section 9.2.3.4 using the a value of guaiacol from Section 10.1.1.4)  

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