Actinomycins purification

Purification has been reported of phenoxazinone synthase, the enzyme which catalyses phenoxazinone formation from 4-methyl-3-hydroxyanthranilic acid (123) in the biosynthesis of actinomycin (124). Two forms of the enzyme, one of high and one of low molecular weight, were isolated. The relative amount of the... 

Kellei U. Schlumbohm W. Purification and characterization of actinomycin synthetase I. a 4-methyl-3-hydroxv-anthraniilic acid-AMP ligase from Streptomyces chrysomallus. J Biol Chem 1992,267 11745-11752. 

Fawaz F, Jones CH. Actinomycin synthesis in Streptomyces ontibiotinu Purification and properties of hydroxYanthranilate-4 mechylcransfetase. ] Biol Chem 1988 263 4602-4606. Jones GH. Combined purification of actinomycin synthetase I and 3-hydroxyanthranilic acid 4-methylttansferase from Streptamyces aruibi rmcus. ] Biol Chem 1993 268 6831-6834. Keller U, Schlumbohm W. Purification and characterization of actinomycin synthetase I. a 4 methy -3-hydroxyanthranilic acid AMP ligase from Streptomjees chrysomallus. J Biol Chem 1992 267 11745-11752. 

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