Acid temperature control interior

For a SERS experiment, the water-soluble cellular fraction from pollen can be obtained as shown in the schematic in Fig. 4.8. About 0.2 mg of freeze-dried pollen, which correspond to a few hundred pollen grains, was suspended in 100 dl water and incubated at room temperature. When water is added to intact pollen, the cellular interior bearing the allergens, but also other proteins, carbohydrates, pigments and nucleic acids is released [91] and hence should be contained in the aqueous supernatant. About 2 pi of the supernatant, which should contain the amount of soluble content of a few individual pollen grains can then be added to 20 pi of a solution of gold nanoparticles. The gold nanoparticles were prepared as described by Lee and Meisel [92]. In the control experiments, the volume of the nanoparticle suspension is replaced by pure water. 

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