Acetylation of the Lignin Preparation

2 Determination of Total Hydroxy) Groups 7.1.2.1 Acetylation of the Lignin Preparation 

Acetic anhydride, freshly distilled, b.p. 138-140°C Acetone, freshly distilled, b.p. 55-56°C 

Dichloromethane, b.p. 39-40°C Ethanol, absolute, b.p. 78-79°C Hydrochloric acid, 2M in water (Note 2) 

Nitrogen, from a cylinder equipped with a pressure regulator and needle valve Phosphorus pentoxide, 98%, powder Pyridine, freshly distilled, b.p. 113-115°C Toluene, freshly distilled, b.p. 110-111 °C 

Note 2. Preferably double-distilled or completely deionized water Procedure 

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