Acetyl procaine

For the analysis of acetyl procaine in heroin samples, Bernauer and Fuchs employed an octadecyl stationary phase in combination with the mobile phase acetonitrile - water (91.5 8.5) containing 8 mg/100 ml tris(hydroxymethyl)-aminomethane (Fig. 7.8). 

Lurie, I.S. McGuiness, K. The quantitation of heroin and selected basic impurities via reversed phase HPLC. II. The analysis of adulterated samples. J.Liq.Chromatogr., 1987,10, 2189-2204 [UV detection electrochemical detection simultaneous acetaminophen, acetylcodeine, acetylmorphine, acetyl-procaine, aminopyrene, amitriptyline, antipyrene, barbital, benztropine, caffeine, cocaine, codeine, diamorphine, diazepam, diphenhydramine, dipyrone, ephedrine, ethylmorphine, lidocaine, meconin, methamphetamine, methap3rrilene, methaqualone, monoacetylmorphine, morphine, nalorphine (IS), niacinamide, noscapine, papaverine, phenacetin, phenmetrazine, phenobarbital, phenolphthsdein, procaine, propanophenone, propoxyphene, pyrilamine, quinidine, quinine, salicylamide, salicylic acid, secobarbital, strychnine, tartaric acid, tetracaine, thebaine, tripelennamine, tropacocaine, vitamin Bj, vitamin B3]... 

Henningsen NC, Cederberg A, Hanson A, Johansson BW. Effects of long-term treatment with procaine amide A prospective smdy with special regard to ANF and SLE in fast and slow acetylators. Acta Med Scand 1975 198 475 82. 

Tri-0-benzoyl-2-chloro-0 -D-xylopyranosyl chloride, the 4-chloroheptose derivative (2), 2-amino-2-deoxy-a-D-galactopyranosyl phosphate, 1,3,4,6-tetra-0-acetyl-2-(A -acetylacetamido)-2-deoxy- -D-galactopyranose, 2-acet-amido-lr/V - (L-aspart-4-oy l)-2- deoxy-jS-D-glucopyranosylamine, and iVn 3-D-gluco-pyranosyl procaine. ... 

Bisulphite interferes with the acetylation and must be eliminated first by addition of a slight excess of iodine in acid solution, followed by de-colorisation with thiosulphate. Procaine will also acetylate under the above conditions but forms a basic derivative which can be extracted in alkaline solution with chloroform as a colourless, viscous oil. 

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