Abnormal Fractionation in Zone Electrophoresis

Fractions run together at paper edges Application faulty Apply a few mm from edge 

Comet formation Adsorption Use a coarser paper raise ambient temperature 

Broad fraction Diffusion Dry starting line before application control total concentration of proteins after optimal duration cease electrophoretic action 

Curved, oblique, jagged, Irregular application Quick backward and for- 

Chemical fixation, which may be combined with staining, is useful and will be considered with the latter. 

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