AAV single polynucleotide chains

C below the Tm of duplex AAV DNA. These duplex linear monomers did form duplex circular monomers and hnear dimers when exposed to annealing conditions. Thus, anneahng of intact linear AAV single polynucleotide chains led to the formation of two types of duplex linear monomers (1) those without cohesive single-stranded termini, and (2) those with cohesive single-stranded termini. 

Fig. 6. Electron micrographs of hydrogen-bonded single-stranded circles formed when purified linear heavy AAV single polynucleotide chains are exposed to annealing conditions. The arrows point to the projections observed on such single-stranded circles...

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