A. thaliana

It has, thus, been demonstrated that redirecting the poly(3HB) biosynthetic pathway from the cytoplasm to the plastid resulted in an approximate 100-fold increase in poly(3HB) production [24]. However, it must be kept in mind that the rate of poly(3HB) biosynthesis in A thaliana leaves was relatively low, since poly(3HB) accumulated progressively over 40-60 days to reach 10-14% of the dry weight, whereas synthesis of starch can reach 17% dry weight for a 12 h photoperiod and seed storage lipids can reach 8% dry weight per day. 

Production of poly(3HB) in the chloroplast of A. thaliana has also been independently demonstrated with similar experiments by the group of Monsanto. They have reported that by using the phaA, phaB, and phaC genes modified for plastid targeting and expressed under the CaMV35S promoter, poly(3HB) levels up to 12-13% dry weight were obtained in A. thaliana shoots. 

The wild type ilvA gene was modified to target the protein to the plastid and expressed in A. thaliana. Transgenic plants showed a 20-fold increase in levels of 2-ketobutyrate as well as a large increase in 2-aminobutyrate, the transaminated product of 2-ketobutyrate [27, 41]. The levels of threonine remained stable whereas isoleucine concentration increased. Constitutive expression of the ilvA protein along with bktB, phaA, and phaC proteins in the plastids of A. thaliana led to the synthesis of poly(3HB-co-3HV) in the range of 0.2 - 0.8 % dry weight, with a HV level between 4-17 mol % [27,41]. Co-expression of the iso-... 

Modulation of the quantity and/or quality of poly(3HAMCL) synthesized in peroxisomes was also achieved by modifying the endogenous fatty acid biosynthetic pathway [58]. For example, expression of the peroxisomal PHA synthase in an A. thaliana mutant deficient in the synthesis of triunsaturated fatty acids [59] resulted in the synthesis of a PHA having an almost complete absence of triunsaturated 3-hydroxyacid monomers [58]. In a different strategy, expression of a fatty acyl-ACP thio esterase in the plastid was combined with the expression of a peroxisomal PHA synthase [58]. Fatty acyl-ACP thioesterases are... 

Subunit S. cerevisiae-MW H. sapiens S. pombe A. thaliana Wheat Domain(s) Function (s) of S. c. 

Enkephalins Promoter and terminator of 2S1 albumin gene of A. thaliana Integrated in 2S1 albumin of A. thaliana and flanked by tryptic cleavage sites A. thaliana (seed) Brassica napus (seed) 2.9% ofTSP, (200 nmol g 1) 50 nmol 3... 

Human creatine kinase -MM MAK33 IgGl Cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle s disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, A. thaliana A. thaliana 2S2 seed storage protein SP + 0.02-0.4% TSP of fresh leaf extract (10-12% TSP of intercellular fluid) 52... 

Zearalenone (mycotoxin) scFv Passive immunization of animals in their feed A. thaliana (ecotype Columbia) Inducible lac No targeting signal - 58... 

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