A -Glutaryl-L-phenylalanine

Challenge Problem. The hydrolysis of N-glutaryl-L-phenylalanine-p-nitroanilide (GPNA) by the enzyme a-chymotrypsin (CT) to form p-nitroaniline and A-glutaryl-L-phenylalanine follows the Michaelis-Menten mechanism in its early. stages. 

The transfer of a-chymotrypsin was also measured. The protein was placed in a pH 7.8 buffer in the internal phase. Substrate, A-glutaryl-L-phenylalanine p-nitroanilide, was placed in the bulk water... 

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