A-Fucosidases

Zhang, J.H., G.Dawes and W.P.Stemmer. (1997) Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. Proc Natl Acad Sci USA, 94, 4504-4509. 

Table 3. Steady-state kinetic constants of wild type and mutants a-fucosidases...

Pisurn sativum (pea) (Fabaceae) a-Fucosidase (20 kDa 4 Cys) No protease inhibitor activity found (but KTI homologue trypsin-resistant) [390, 391]... 

Svensson SCT, Thiem J. Purification of a-fucosidase by C-glycosylic affinity chromatography, and the enzymic synthesis of a-fucosyl disaccharides Carbohydr. Res. 1990 200 391-402. 

Deoxy-6-C-(2,5- dihydroxyhexyD-DMDP moderate inhibitor of amyloglucosidase and weak inhibitor of a-fucosidase"... 

Deoxynojirimycin (DNJ) potent inhibitor of a-glucosidases (including trehalase, invertase, sucrase, maltase, isomaltase), more weakly inhibitory to Glucosidase I and II, and p-glucosidases, also (x-mannosidases, a-fucosidase, a- and p-galactosidases (including lactase) - "... 

Hyacinthacine Aj Potent inhibitor of lactase, moderate inhibitor of amyloglucosidase and a-fucosidase and a poor inhibitor of a- and P-glucosidases and P-mannosidase ... 

Hyacinthacine B3 good inhibitor of lactase and amyloglucosidase and poor inhibitor of a-fucosidase ... 

Fucosidosis, being a so-called mucolipidosis, can be grouped among the mucopolysaccharidoses. It is due to a-fucosidase deficiency (localized on chromosome Ip 34). The storage products are deposited in the form of granular or lamellar inclusions in the lysosomes. This autosomal recessive disorder was first observed by P. Durand et al. in 1967 and clarified by F. Van Hoof et al. in 1968. Besides hepatomegaly, splenomegaly may also sometimes occur. 

Carpintero, M, Bastida, A, Garcia-Junceda, E, Jimenez-Barbero, J, Fernandez-Mayoralas, A, Synthesis of carba- and C-fucopyranosides and their evaluation as a-fucosidase inhihitors-analysis of an unusual conformation adopted by an amino-C-fucopyranoside, Eur. J. Org. Chem., 4, 4127-4135, 2001. 

For instance a-fucosides and a-sialosides, which are both difficult to produce by means of classical carbohydrate chemistry, have been synthesized using the corresponding nitrophenyl glycoside donors by applying pig liver a-fucosidase [59] and the sialidase from Vibrio cholerae [60], respectively. 

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