Kaiser test


The methods for the detection of amine functional groups are well established. For example the Kaiser test can be used to detect the presence of amine groups on resins (blue colour is observed). In the Kaiser test, two reagents are prepared. Reagent 1 comprises of a mixture of two solutions A and B. A is a solution of phenol in absolute ethanol (40g of phenol in 10 mL of absolute ethanol, followed by treatment of this clear solution with 4g of Amberlite mixed bed resin MB-3 for 45 mins. The solution is then filtered.). Solution B is made up of 65 mg of KCN in 100 mL water 2 mL of this solution is diluted to KK) mL of freshly distilled pyridine. TTie solution is then stirred with 4 g of Amberlite mixed-bed resin MB-3 and filtered. Solutions A and B are then mixed. Reagent 2 is a solution of ninhydrin (2.5g) in absolute ethanol (50 mL). For a qualitative Kaiser test, 6 drops of reagent 1 and 2 drops of reagent 2 are added to the well washed dried resin (2-5 mg) and mixed, followed by heating to 100°C for 4-6 min. A method for the quantitative determination of amino groups using this test has also been reported [Sarin et al. Anal Biochem 117 147 ]98]]. It is however known that the Kaiser test does not give a positive test with a secondary amino acid such as proline or some unnatural amino acids. In addition some deprotected amino acids (Ser, Asn, Asp) do not show the expected intense blue colour typical of free primary amino groups.  [c.76]

A. Kasahara, T. Izumi, M. Yodono, R. Saito, T. Takeda, and T. Sugawara, Bull. Chem. Soc. Jpn., 46, 1220 (1973).  [c.115]

Forensic Applications UV/Vis molecular absorption is routinely used in the analysis of narcotics and for drug testing. One interesting forensic application is the determination of blood alcohol using the Breathalyzer test. In this test a 52.5-mL breath sample is bubbled through an acidified solution of KaCraOy. Any ethanol present in the breath sample is oxidized by the dichromate, producing acetic acid and Cr as products. The concentration of ethanol in the breath sample is determined from the decrease in absorbance at 440 nm where the dichromate ion absorbs. A blood alcohol content of 0.10%, which is the legal limit in most states, corresponds to 0.025 mg of ethanol in the breath sample.  [c.398]

Naito, T., Oshima, Y., Yoshikawa,T., Kasahara, A., Dohmori.R., Nakai, Y. and Tsukada, W. South African Patent Application 67/4936 January 19,1968 assigned to Dalichi Seiyaku Company Limited, Japan  [c.564]


See pages that mention the term Kaiser test : [c.2965]    [c.119]   
Purification of laboratory chemicals (2003) -- [ c.76 ]